Shoulder pain is one of the most common complaints among volleyball players, with the supraspinatus muscle tendon being one of the most susceptible to injury in this type of sport. The movement of the shoulder joint is highly demanding, and the energy involved is relatively high, which affects and exceeds its physiological limits, resulting in rotator cuff tendinopathies, which include the supraspinatus muscle tendon. Ultrasound, a non-invasive technique, is essential for detailed musculoskeletal injury evaluation. This study aimed to assess the supraspinatus muscle tendon's morphology through ultrasound in a population that practices volleyball, compared to a non-practitioner group. We specifically considered gender, age, height, dominant upper limb, and volleyball practice as key factors.