With a prevalence of approximately 3.8% in the general population, carpal tunnel syndrome (CTS) is the most common entrapment neuropathy, posing a global health burden. Compression of the median nerve at the level of the wrist (carpal tunnel) results in dysesthesias, pain, and, in more severe cases, atrophy of median nerve innervated muscles. Essential for the diagnosis of CTS are a precise medical history and clinical examination, including Phalen’s test (maximal flexion of the wrists for one minute) and Hoffmann-Tinel sign (paresthesia along the course of the median nerve after tapping on the wrist). Further examinations include nerve conduction studies and high-resolution ultrasound (HRUS). HRUS depicts pathological changes in the median nerve, such as morphological alterations (mainly an increase in the cross-sectional area proximal to the carpal tunnel) and hypervascularization. Additionally, surrounding soft tissue (flexor tendons, transverse carpal ligament, nerves, vessels) can also be assessed in detail. Mild to moderate CTS can be treated conservatively, whereas more severe cases require surgical decompression. Although open and endoscopic surgical approaches for carpal tunnel release (CTR) are available,  surgical complications include pillar pain, scar tenderness, recurrent CTS trigger finger and Depuytren’s contracture.Thread carpal tunnel release is the most recent of a number of minimally invasive ultrasound-guided carpal tunnel releases techniques. The purpose of this study was to assess the transection of the transverse carpal ligament and damage to surrounding structures after ultrasound guided thread carpal tunnel release performed on anatomical specimens.