Methods
1. Study design
This is a observational cohort study aimed at evaluating the anatomical variations in the localization and morphology of the peroneus brevis tendon using magnetic resonance imaging (MRI).
2. Setting
Ankle MRI scans performed on a 3T scanner between 2019 and 2024 at our center were re-evaluated. Recruitment and data collection occurred during this period, with patient scans analyzed retrospectively.
3. Participants
Patients who underwent MRI of the ankle at our center between 2019 and 2024 were included. Exclusion criteria included abnormal signal from peroneus tendons, lateral malleolar symptoms, history of peroneus tendon pathology, prior surgery, inflammatory conditions, or tumors.
4. Outcomes
Primary outcome: localization of the peroneus brevis tendon relative to the peroneus longus.
Secondary outcomes: the shape of the tendon on the axial cross section and its cross-sectional area (in mm2).
Exposures: peroneus brevis tendon position at the level of the lateral malleolus.
Potential confounders: age, sex, and side of the body.
5. Data sources and measurements
MRI transverse slices (proton density-weighted) 1 cm above the lateral malleolus apex were identified by two independent radiologists. Discrepancies were resolved by consensus. The slices were analyzed using ImageJ, where the outlines of the peroneus brevis, peroneus longus, and fibula were segmented by musculoskeletal radiologist with 10 years’ experience to obtain coordinates (X, Y) and cross-sectional areas of the tendons.
6. Sample size
A sample size of 218 patients was selected to ensure a statistical power greater than 0.8 (alfa less than 0.05), effect size low, based on expected differences in tendon areas between relative positions and shapes.
7. Quantitative analysis
Tendon area (in mm²) as a continuous variable was compared between tendon shapes using ANOVA.
8. Statistical analysis
Chi-square tests were used to compare categorical variables, while ANOVA or Kruskal-Wallis tests were used for continuous variables based on data distribution. Subgroup analysis was performed by age group and sex to explore potential effect modifiers.
9. Funding for this study
Stiftelsen Tornspiran (934 and 1036).